Mass propagation of Solanum surattense Bum. using direct adventitious shoot organogenesis from internode

An efficient and reproducible procedure was established for direct shoot regeneration of Solanum surattense Bum. using internodal explants. The shoot regeneration efficiency on Murashige and Skoog (MS) medium supplemented with 6benzylaminopurine (BAP) was superior in compared to 6furfurylaminopurine (Kn). The highest mean number of shoots per explant was recorded on MS medium containing 0.5 mgl BAP. BAP alone was more effective for shoot regeneration than combination of BAP and -naphthaleneacetic acid (NAA). The number of shoot per explant was highest in the 4 subculture and then it was remained stable up to 6 subculture. The shoot number drastically reduced from 7 subculture when explants were repeated subculturing up to 8 subculture. Hundred percent adventitious shoots were rooted successively on half strength MS medium fortified with 0.05 mgl NAA. At about 90% of the in vitro-propagated plantlets survived when they were transferred to ex vitro conditions for acclimatization.